Structural and Mechanistic Studies of Gene Regulation
The main focus of Mark Glover's research is to understand, in structural detail, the molecular processes by which gene expression and DNA repair are regulated. Our lab uses a combination of biochemical and structural approaches to probe mechanistic aspects of several distinct systems.
The DNA damage response and DNA repair
Phosphorylation regulates the DNA damage response

All cells have evolved elaborate mechanisms to deal with damage to the genetic information. These systems not only involve an array of DNA repair enzymes that can handle the diversity of damage that can occur, but also protein signaling systems that not only coordinate the recognition and repair of the damage, but also modulate DNA replication, transcription and the cell cycle in response to the damage. Taken together, these systems provide genetic stability and protect cells against transitions to a cancerous state. Our lab is interested in understanding the mechanisms that underlie DNA repair and DNA damage signaling.

Regulation of bacterial conjugation
EM of conjugation

Bacterial pathogens can rapidly acquire antibiotic resistance and virulence through an uncanny ability to share DNA between cells through the process of bacterial conjugation. Conjugation involves the transfer of plasmid DNA between cells through a protein filament termed the pilus which links the two cells. We are interested in understanding the systems that regulate the conjugative transfer of the F family of plasmids.

Regulation of eukaryotic gene expression

We are interested in understanding the principles that underlie the specific recognition of DNA by complex eukaryotic transcription factors.