Current Members
Ross Edwards
Ross Edwards
Research Associate, 2000

FinO-RNA interactions.

Hodge, C. D., Ismail, I. H., Edwards, R. A., Hura, G. L., Xiao, A. T., Tainer, J. A., Hendzel, M. J., Glover, J. N. (2016) RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem. 291(18):9396-410. PDF
Awards

AHFMR Fellowship (2000-2002)

Publications
20 during tenure in Glover's laboratory, 20 total.
Hodge, C. D., Ismail, I. H., Edwards, R. A., Hura, G. L., Xiao, A. T., Tainer, J. A., Hendzel, M. J., Glover, J. N. (2016) RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem. 291(18):9396-410. PDF
Hodge, C. D., Edwards, R. A., Markin, C. J., McDonald, D., Pulvino, M., Huen, M. S., Zhao, J., Spyracopoulos, L., Hendzel, M. J., Glover, J. N. (2015) Covalent Inhibition of Ubc13 Affects Ubiquitin Signaling and Reveals Active Site Elements Important for Targeting. ACS Chem Biol. 10(7):1718-28. PDF
Glover, J. N., Chaulk, S. G., Edwards, R. A., Arthur, D., Lu, J., Frost, L. S. (2015) The FinO family of bacterial RNA chaperones. Plasmid. 78(79-87) PDF
White, E. R., Sun, L., Ma, Z., Beckta, J. M., Danzig, B. A., Hacker, D. E., Huie, M., Williams, D. C., Edwards, R. A., Valerie, K., Glover, J. N., Hartman, M. C. (2015) Peptide library approach to uncover phosphomimetic inhibitors of the BRCA1 C-terminal domain. ACS Chem Biol. 10(5):1198-208. PDF
Peng, Y., Lu, J., Wong, J. J., Edwards, R. A., Frost, L. S., Glover, J. N. (2014) Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM. J Mol Biol. 426(22):3783-95. PDF
Leung, C. C., Sun, L., Gong, Z., Burkat, M., Edwards, R., Assmus, M., Chen, J., Glover, J. N. (2013) Structural insights into recognition of MDC1 by TopBP1 in DNA replication checkpoint control. Structure. 21(8):1450-9. PDF
Campbell, S. J., Edwards, R. A., Leung, C. C., Neculai, D., Hodge, C. D., Dhe-Paganon, S., Glover, J. N. (2012) Molecular insights into the function of RING finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation. J Biol Chem. 287(28):23900-10. PDF
Arthur, D. C., Edwards, R. A., Tsutakawa, S., Tainer, J. A., Frost, L. S., Glover, J. N. M. (2011) Mapping interactions between the RNA chaperone FinO and its RNA targets. Nucl. Acids Res. Advanced Online PDF
Wong, J. J., Lu, J., Edwards, R. A., Frost, L. S., Glover, J. N. M. (2011) Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM. Nucleic Acids Research Epub ahead of print
Chaulk, S. G., Smith Frieday, M. N., Arthur, D. C., Culham, D. E., Edwards, R. A., Soo, P., Frost, L. S., Keates, R. A., Glover, J. N. M. (2011) ProQ Is an RNA Chaperone that Controls ProP Levels in Escherichia coli. Biochemistry. Epub ahead of print
Thede, G. L., Arthur, D. C., Edwards, R. A., Buelow, D. R., Wong, J. L., Raivio, T. L., Glover, J. N. M. (2011) Structure of the periplasmic stress response protein CpxP. J Bacteriol. 2011 Feb 11. [Epub ahead of print]
Chaulk, S., Lu, J., Tan, K., Arthur, D. C., Edwards, R. A., Frost, L. S., Joachimiak, A., Glover, J. N. M. (2010) N. meningitidis 1681 is a member of the FinO family of RNA chaperones. RNA Biology 7(6):112-119.
Campbell, S. J., Edwards, R. A. and Glover, J. N. M. (2010) Comparison of the structures and peptide binding specificities of the BRCT domains of MDC1 and BRCA1. Structure 18:167-176. PDF
Edwards, R. A., Lee, M. S., Tsutakawa, S. E., Williams, R. S., Nazeer, I., Kleiman, F.E., Tainer, J. A. and Glover, J. N. (2008) The BARD1 C-terminal domain structure and interactions with polyadenylation factor, CstF-50. Biochemistry 47(44):11446-11456. PDF
Lu, J., Wong, J. J. W., Edwards, R. A., Manchak, J., Frost, L. S. and Glover, J. N. M. (2008) Structural basis of specific TraD-TraM recognition during F plasmid-mediated bacterial conjugation. Molecular Microbiology 70(1):89-99. PDF
Lu, J., Edwards, R. A., Wong, J. J. W., Manchak, J. Scott, P. G., Frost, L. S. and Glover, J. N. M. (2006) Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM. EMBO Journal 25(12):2930-2939. PDF
Schellenberg, M. J., Edwards, R. A., Ritchie, D. B., Kent, O. A., Golas, M. M., Stark, H., Lührmann, R., Glover, J. N. M. and MacMillan, A. M. (2006) Crystal structure of a core spliceosomal protein interface. PNAS 103(5):1266-1271. PDF
Lee, M. S., Edwards, R. A., Thede, G. L. and Glover, J. N. M. (2005) Structure of the BRCT repeat domain of MDC1 and its specificity for the free COOH-terminal end of the gamma-H2AX histone tail. JBC 280(37):32053-32056. PDF
Arthur, D. C., Ghetu, A. F., Gubbins, M. J., Edwards, R. A., Frost, L. S. and Glover, J. N. M. (2003) FinO is an RNA chaperone that facilitates sense-antisense RNA interactions. EMBO J 22(23):6346-6355. PDF
Moraes, T. F., Edwards, R. A., McKenna, S., Pastushok, L., Xiao, W., Glover, J. N. M. and Ellison, M. J. (2001) Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13. Nature Structure Biology 8:669-673. PDF
Abstracts & Invited Seminars
Zahra Havalishahriari
Zahra Havalishahriari
PhD Candidate, 2010

Faculty of Medicine and Dentistry 75th Anniversary Award (2011)

I am working on polynucleotide kinase/phosphatase (PNKP) which has an essential role in DNA repair pathways. I am focusing on structural studies of PNKP substrate binding. In addition, I am characterizing inhibitors for PNKP which can be developed into novel cancer drugs that sensitize tumor cells to radio- and chemotherapy.

Coquelle, N., Havali-Shahriari, Z., Bernstein, N., Green, R., Glover, J. N. (2011) Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates. Proc Natl Acad Sci U S A. 108(52):21022-7. PDF
Awards

Faculty of Medicine and Dentistry 75th Anniversary Award (2011)

Publications
One during tenure in Glover's laboratory, one total.
Coquelle, N., Havali-Shahriari, Z., Bernstein, N., Green, R., Glover, J. N. (2011) Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates. Proc Natl Acad Sci U S A. 108(52):21022-7. PDF
Abstracts & Invited Seminars
Curtis Hodge
Curtis Hodge
PhD Candidate, 2010

AHFMR Studentship (2011-2016)

Hodge, C. D., Spyracopoulos, L., Glover, J. N. (2016) Ubc13: the Lys63 ubiquitin chain building machine. Oncotarget. 7(39):64471-64504. PDF
Awards

AHFMR Studentship (2011-2016)

NSERC Alexander Graham Bell Canada Graduate Scholarship (2010)

Publications
Five during tenure in Glover's laboratory, six total.
Hodge, C. D., Spyracopoulos, L., Glover, J. N. (2016) Ubc13: the Lys63 ubiquitin chain building machine. Oncotarget. 7(39):64471-64504. PDF
Hodge, C. D., Ismail, I. H., Edwards, R. A., Hura, G. L., Xiao, A. T., Tainer, J. A., Hendzel, M. J., Glover, J. N. (2016) RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem. 291(18):9396-410. PDF
Hodge, C. D., Edwards, R. A., Markin, C. J., McDonald, D., Pulvino, M., Huen, M. S., Zhao, J., Spyracopoulos, L., Hendzel, M. J., Glover, J. N. (2015) Covalent Inhibition of Ubc13 Affects Ubiquitin Signaling and Reveals Active Site Elements Important for Targeting. ACS Chem Biol. 10(7):1718-28. PDF
Rout, M. K., Hodge, C. D., Markin, C. J., Xu, X., Glover, J. N., Xiao, W., Spyracopoulos, L. (2014) Stochastic gate dynamics regulate the catalytic activity of ubiquitination enzymes. J Am Chem Soc. 136(50):17446-58. PDF
Campbell, S. J., Edwards, R. A., Leung, C. C., Neculai, D., Hodge, C. D., Dhe-Paganon, S., Glover, J. N. (2012) Molecular insights into the function of RING finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation. J Biol Chem. 287(28):23900-10. PDF
Hodge, C. D., Wang, H., Sun, G. (2010) Phylogenetic analysis of the maternal genome of tetraploid StStYY Elymus (Triticeae: Poaceae) species and the monogenomic Triticeae based on rps16 sequence data Plant Science 463-468
Abstracts & Invited Seminars
Sheraz Khan
Sheraz Khan
X-ray Technician, 2010

Awards
Publications
0 during tenure in Glover's laboratory, 0 total.
Abstracts & Invited Seminars
Jin Kim
Jin Kim
Masters Student, 2014

Awards
Publications
0 during tenure in Glover's laboratory, 0 total.
Abstracts & Invited Seminars
Jun Lu
Jun Lu
Research Associate, 2004

I study proteins involved in bacterial conjugation, a process responsible for diversifying bacterial genomes and quick dissemination of antibiotic resistance. Specifically, we want to understand how a single stranded DNA is processed and transferred in response to an unidentified mating signal generated by intimate donor-recipient cell contact.

Peng, Y., Lu, J., Wong, J. J., Edwards, R. A., Frost, L. S., Glover, J. N. (2014) Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM. J Mol Biol. 426(22):3783-95. PDF
Awards

AHFMR Fellowship (2006-2007)

CIHR Fellowship (2006-2009)

Leaders in Biochemistry Postdoctoral Fellows Award (2007)

Leaders in Biochemistry Postdoctoral Fellows Award (2010)

Publications
Eight during tenure in Glover's laboratory, eight total.
Peng, Y., Lu, J., Wong, J. J., Edwards, R. A., Frost, L. S., Glover, J. N. (2014) Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM. J Mol Biol. 426(22):3783-95. PDF
Lu, J., Wu, R., Adkins, J. N., Joachimiak, A., Glover, J. N. (2014) Crystal structures of the F and pSLT plasmid TraJ N-terminal regions reveal similar homodimeric PAS folds with functional interchangeability. Biochemistry. 53(36):5810-9. PDF
Lu, J., Peng, Y., Arutyunov, D., Frost, L. S., Glover, J. N. (2012) Error-prone PCR mutagenesis reveals functional domains of a bacterial transcriptional activator, TraJ. J Bacteriol. () PDF
Wong, J. J., Lu, J., Edwards, R. A., Frost, L. S., Glover, J. N. M. (2011) Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM. Nucleic Acids Research Epub ahead of print
Chaulk, S., Lu, J., Tan, K., Arthur, D. C., Edwards, R. A., Frost, L. S., Joachimiak, A., Glover, J. N. M. (2010) N. meningitidis 1681 is a member of the FinO family of RNA chaperones. RNA Biology 7(6):112-119.
Lu, J., den Dulk-Ras, A., Hooykaas, P. J. and Glover, J. N. M. (2009) Agrobacterium tumefaciens VirC2 enhances T-DNA transfer and virulence through its C-terminal ribbon-helix-helix DNA-binding fold. PNAS 106(24):9643-9648. PDF
Lu, J., Wong, J. J. W., Edwards, R. A., Manchak, J., Frost, L. S. and Glover, J. N. M. (2008) Structural basis of specific TraD-TraM recognition during F plasmid-mediated bacterial conjugation. Molecular Microbiology 70(1):89-99. PDF
Lu, J., Edwards, R. A., Wong, J. J. W., Manchak, J. Scott, P. G., Frost, L. S. and Glover, J. N. M. (2006) Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM. EMBO Journal 25(12):2930-2939. PDF
Abstracts & Invited Seminars
Lucy Sun
Lucy Sun
PhD Candidate, 2011

I study the structure and function of the Bmi1-Ring1B polycomb group ubiquitin ligase complex.

White, E. R., Sun, L., Ma, Z., Beckta, J. M., Danzig, B. A., Hacker, D. E., Huie, M., Williams, D. C., Edwards, R. A., Valerie, K., Glover, J. N., Hartman, M. C. (2015) Peptide library approach to uncover phosphomimetic inhibitors of the BRCA1 C-terminal domain. ACS Chem Biol. 10(5):1198-208. PDF
Awards
Publications
Two during tenure in Glover's laboratory, two total.
White, E. R., Sun, L., Ma, Z., Beckta, J. M., Danzig, B. A., Hacker, D. E., Huie, M., Williams, D. C., Edwards, R. A., Valerie, K., Glover, J. N., Hartman, M. C. (2015) Peptide library approach to uncover phosphomimetic inhibitors of the BRCA1 C-terminal domain. ACS Chem Biol. 10(5):1198-208. PDF
Leung, C. C., Sun, L., Gong, Z., Burkat, M., Edwards, R., Assmus, M., Chen, J., Glover, J. N. (2013) Structural insights into recognition of MDC1 by TopBP1 in DNA replication checkpoint control. Structure. 21(8):1450-9. PDF
Abstracts & Invited Seminars
Alumni
Danny Aceytuno
Danny Aceytuno
PhD Candidate, 2006

Queen Elizabeth II Graduate Scholarship - Doctoral Level (2009-2010, 2010-2011)

My work focuses on the polynucleotide kinase/phosphatase (PNKP)/XRCC4/DNA Ligase IV non-homologous end joining (NHEJ) DNA repair complex. This assembly is responsible for processing abnormal DNA end chemistries would otherwise preclude the final repair step of NHEJ in double-strand break repair. I study the structural dynamics and enzymatic activities of this complex.

Siribal, S., Weinfeld, M., Karimi-Busheri, F., Glover, J. N. M., Bernstein, N. K., Aceytuno, D., Chavalitshewinkoon-Petmitr, P. (2011) Molecular characterization of Plasmodium falciparum putative polynucleotide kinase/phosphatase. Mol Biochem Parasitol. 2011 Nov;180(1):1-7
Awards

Queen Elizabeth II Graduate Scholarship - Doctoral Level (2009-2010, 2010-2011)

Graduate Students’ Association Research Communication Outreach Award (2011)

Government of Alberta Graduate Citizenship Award for Student Service (2009)

Biological Sciences Undergraduate Research Award (2006)

Publications
Two during tenure in Glover's laboratory, two total.
Siribal, S., Weinfeld, M., Karimi-Busheri, F., Glover, J. N. M., Bernstein, N. K., Aceytuno, D., Chavalitshewinkoon-Petmitr, P. (2011) Molecular characterization of Plasmodium falciparum putative polynucleotide kinase/phosphatase. Mol Biochem Parasitol. 2011 Nov;180(1):1-7
Weinfeld, M., Mani, R. S., Abdou, I., Aceytuno, R. D., Glover, J. N. M. (2011) Tidying up loose ends: the role of polynucleotide kinase/phosphatase in DNA strand break repair. Trends Biochem Sci. Epub ahead of print
Abstracts & Invited Seminars

2010 Alberta Cancer Foundation Research Meeting - Cancer Research in Alberta: From Bench to Society, November 2010, Banff, AB (R. Daniel Aceytuno, Ross A. Edwards, Ryan Au, J.N. Mark Glover): “Structural dynamics and enzymatic studies of the NHEJ XRCC4/DNA Ligase IV/polynucleotide kinase-phosphatase DNA repair complex.”

2010 Alberta Cancer Foundation Research Meeting - Cancer Research in Alberta: From Bench to Society, November 2010, Banff, AB (R. Daniel Aceytuno, Ross A. Edwards, Ryan Au, J.N. Mark Glover): “Structural dynamics and enzymatic studies of the NHEJ XRCC4/DNA Ligase IV/polynucleotide kinase-phosphatase DNA repair complex.”

Alberta Genomic Instability and Aging Conference, June 2010, Calgary, AB (R. Daniel Aceytuno, Ross A. Edwards, Ryan Au, J.N. Mark Glover): “Structural dynamics of the NHEJ XRCC4/DNA Ligase IV/polynucleotide kinase-phosphatase DNA repair complex.”

Ninth Annual Structural Biology of DNA Repair Program Project Workshop, March 2010, Berkeley, CA (R. Daniel Aceytuno, Ross A. Edwards, Ryan Au, J.N. Mark Glover): “Small-angle X-ray scattering models of the NHEJ XRCC4/DNA Ligase IV and XRCC4/DNA Ligase IV/polynucleotide kinase phosphatase DNA repair complexes”

2009 Research Meeting of the Alberta Cancer Research Institute - Celebrating Excellence in Cancer Research in Alberta, November 2009, Banff, AB (R. Daniel Aceytuno, Ross A. Edwards, Ryan Au, J.N. Mark Glover): “Structural studies of the non-homologous end joining XRCC4/DNA Ligase IV/polynucleotide kinase end-processing assembly by small-angle X-ray scattering”

Southern Alberta Cancer Research Institute Genomic Instability and Aging Conference, June 2009, Calgary, AB (R. Daniel Aceytuno, Ryan Au, Nina K. Bernstein, J.N. Mark Glover): “Structural studies of the XRCC4/DNA Ligase IV/polynucleotide kinase end-processing assembly in non-homologous end joining”

3rd US/EU International Conference on Endogenous Genome Damage and Repair, February 2009, Galveston, TX (R. Daniel Aceytuno, Ryan Au, Nina K. Bernstein, J.N. Mark Glover): “Structural studies of the XRCC4/DNA Ligase IV/polynucleotide kinase end-processing assembly in non-homologous end joining”

2nd Annual Alberta Cancer Research Institute Research Meeting - Building Bridges and Closing Gaps: Towards an Integrated Approach to Cancer Research, November 2008, Banff, AB (R. Daniel Aceytuno, Ryan Au, Nina K. Bernstein, J.N. Mark Glover): “Structural studies of the XRCC4/DNA Ligase IV/polynucleotide kinase end-processing assembly in non-homologous end joining”

Nina Bernstein
Nina Bernstein
Research Associate, 2000-2010

Mammalian Polynucleotide Kinase/Phosphatase (PNKP) is a key enzyme in the repair of DNA strand breaks. We have elucidated the structure and minimal substrate preferences of PNKP. We are working toward a detailed structural understanding of how PNKP interacts with its substrates with the aim of applying this knowledge to inhibitor design.

Coquelle, N., Havali-Shahriari, Z., Bernstein, N., Green, R., Glover, J. N. (2011) Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates. Proc Natl Acad Sci U S A. 108(52):21022-7. PDF
Awards

NCIC Fellowship (2002-2004)

NCIC Terry Fox Fellowship

Publications
Seven during tenure in Glover's laboratory, seven total.
Coquelle, N., Havali-Shahriari, Z., Bernstein, N., Green, R., Glover, J. N. (2011) Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates. Proc Natl Acad Sci U S A. 108(52):21022-7. PDF
Bernstein, N. K., Hammel, M., Mani, R. S., Weinfeld, M., Pelikan, M., Tainer, J. A. and Glover, J. N. M. (2009) Mechanism of DNA substrate recognition by the mammalian DNA repair enzyme, polynucleotide kinase. Nucleic Acids Res. 37(18):6161-6173. PDF
Bernstein, N. K., Karimi-Busheri, F., Rasouli-Nia, A., Mani, R., Dianov, G., Glover, J. N. and Weinfeld, M. (2008) Polynucleotide kinase as a potential target for enhancing cytotoxicity by ionizing radiation and topoisomerase I inhibitors. Anticancer Agents Med Chem. (Review). 8(4):358-367.
Ulke-Lemée, A., Trinkle-Mulcahy, L., Chaulk, S., Bernstein, N. K., Morrice, N., Glover, M., Lamond, A. I. and Moorhead, G. B. G. (2007) The nuclear PP1 interacting protein ZAP3 (ZAP) is a putative nucleoside kinase that complexes with SAM68, CIA, NF110/45, and HNRNP-G. BBA - Proteins and Proteomics 1774:1339-1350. PDF
Williams, R. S., Bernstein, N. K., Lee, M. S., Rakovszky, M. L., Cui, D., Green, R., Weinfeld, M. and Glover, J. N. M. (2005) Structural basis for phosphorylation-dependent signaling in the DNA-damage response. Biochemistry and Cell Biology 83(6):721-727. PDF
Bernstein, N. K., Williams, R. S., Rakovszky, M. L., Cui, D., Green, R., Karimi-Busheri, F., Mani, R. S., Galicia, S. Koch, C. A., Cass, C. E., Durocher, D., Weinfeld, M. and Glover, J. N. M. (2005) The molecular architecture of the mammalian DNA repair enzyme, polynucleotide kinase. Molecular Cell 17(5):657-670. PDF
Fanta, M., Zhang, H., Bernstein, N., Glover, M., Karimi-Busheri, F. and Weinfeld, M. (2001) Production, characterization, and epitope mapping of monoclonal antibodies against human polydeoxyribonucleotide kinase. Hybridoma 20:237-242.
Abstracts & Invited Seminars
Mike Burkat
Mike Burkat
Masters Student, 2011-2013

I am trying to acquire the structures of replicative stress response proteins by crystallography, and characterize them biochemically.

Leung, C. C., Sun, L., Gong, Z., Burkat, M., Edwards, R., Assmus, M., Chen, J., Glover, J. N. (2013) Structural insights into recognition of MDC1 by TopBP1 in DNA replication checkpoint control. Structure. 21(8):1450-9. PDF
Awards
Publications
One during tenure in Glover's laboratory, one total.
Leung, C. C., Sun, L., Gong, Z., Burkat, M., Edwards, R., Assmus, M., Chen, J., Glover, J. N. (2013) Structural insights into recognition of MDC1 by TopBP1 in DNA replication checkpoint control. Structure. 21(8):1450-9. PDF
Abstracts & Invited Seminars
Stephen Campbell
Stephen Campbell
PhD, 2005-2012

Faculty of Medicine and Dentistry 75th Anniversary Award (2010)

I study different aspects of the DNA damage response, including homologous recombination and cell growth regulation. Current research includes screening and characterization of potential small molecule inhibitors of BRCA1 that may prove to be useful therapeutically, as well as structural studies of K63 E3 ubiquitin ligases involved in checkpoint control, such as CHFR.

Campbell, S. J., Edwards, R. A., Leung, C. C., Neculai, D., Hodge, C. D., Dhe-Paganon, S., Glover, J. N. (2012) Molecular insights into the function of RING finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation. J Biol Chem. 287(28):23900-10. PDF
Awards

Faculty of Medicine and Dentistry 75th Anniversary Award (2010)

Queen Elizabeth Doctoral Award (2009-2010)

Publications
Three during tenure in Glover's laboratory, three total.
Campbell, S. J., Edwards, R. A., Leung, C. C., Neculai, D., Hodge, C. D., Dhe-Paganon, S., Glover, J. N. (2012) Molecular insights into the function of RING finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation. J Biol Chem. 287(28):23900-10. PDF
Campbell, S. J., Edwards, R. A. and Glover, J. N. M. (2010) Comparison of the structures and peptide binding specificities of the BRCT domains of MDC1 and BRCA1. Structure 18:167-176. PDF
Yuan, Z., Kumar, E. A., Campbell, S. J., Palermo, N. Y., Kizhake, S., Glover, J. N. M., Natarajan, A. (2011) Exploiting the P-1 pocket of BRCT domains toward a structure guided inhibitor design. ACS Med Chem Lett. Oct 13;2(10):764-767
Abstracts & Invited Seminars
Steve Chaulk
Steve Chaulk
Research Associate, 2002-2010

Canadian Institutes of Health Research Fellowship (2004-2007)

I am interested in miRNA mediated gene regulation and the role of pri-miRNA tertiary structure in miRNA biogenesis. Approximately 50% of miRNAs are expressed as a pri-miRNA cluster containing 2-8 miRNAs per transcript. I am investigating what role the clustering of miRNAs has on their expression and subsequent gene regulation at the proteome level.

Glover, J. N., Chaulk, S. G., Edwards, R. A., Arthur, D., Lu, J., Frost, L. S. (2015) The FinO family of bacterial RNA chaperones. Plasmid. 78(79-87) PDF
Awards

Canadian Institutes of Health Research Fellowship (2004-2007)

Natural Sciences and Engineering Research Council Postdoctoral Fellowship (2002-2004)

Walter C. Sumner Fellowship (2001)

John C. Bunting Graduate Prize in Organic Chemistry (2000)

Ontario Graduate Scholarship (1998-2001)

Publications
Seven during tenure in Glover's laboratory, 15 total.
Glover, J. N., Chaulk, S. G., Edwards, R. A., Arthur, D., Lu, J., Frost, L. S. (2015) The FinO family of bacterial RNA chaperones. Plasmid. 78(79-87) PDF
White, E. R., Sun, L., Ma, Z., Beckta, J. M., Danzig, B. A., Hacker, D. E., Huie, M., Williams, D. C., Edwards, R. A., Valerie, K., Glover, J. N., Hartman, M. C. (2015) Peptide library approach to uncover phosphomimetic inhibitors of the BRCA1 C-terminal domain. ACS Chem Biol. 10(5):1198-208. PDF
Chaulk, S. G., Xu, Z., Glover, M. J., Fahlman, R. P. (2014) MicroRNA miR-92a-1 biogenesis and mRNA targeting is modulated by a tertiary contact within the miR-17~92 microRNA cluster. Nucleic Acids Res. 42(8):5234-44. PDF
Chaulk, S. G., Thede, G. L., Kent, O. A., Xu, Z., Gesner, E., Veldhoen, R. A., Khanna, S. K., Goping, I. S., Macmillan, A. M., Mendell, J. T., Young, H., Fahlman, R. P., Glover, J. N. M. (2011) Role of pri-miRNA tertiary structure in miR-17~92 miRNA biogenesis. RNA Biology 8(6). [Epub ahead of print]
Chaulk, S. G., Smith Frieday, M. N., Arthur, D. C., Culham, D. E., Edwards, R. A., Soo, P., Frost, L. S., Keates, R. A., Glover, J. N. M. (2011) ProQ Is an RNA Chaperone that Controls ProP Levels in Escherichia coli. Biochemistry. Epub ahead of print
Chaulk, S., Lu, J., Tan, K., Arthur, D. C., Edwards, R. A., Frost, L. S., Joachimiak, A., Glover, J. N. M. (2010) N. meningitidis 1681 is a member of the FinO family of RNA chaperones. RNA Biology 7(6):112-119.
Ulke-Lemée, A., Trinkle-Mulcahy, L., Chaulk, S., Bernstein, N. K., Morrice, N., Glover, M., Lamond, A. I. and Moorhead, G. B. G. (2007) The nuclear PP1 interacting protein ZAP3 (ZAP) is a putative nucleoside kinase that complexes with SAM68, CIA, NF110/45, and HNRNP-G. BBA - Proteins and Proteomics 1774:1339-1350. PDF
Chaulk SG, MacMillan AM. (2007) Synthesis of oligo-RNAs with photocaged adenosine 2'-hydroxyls. Nat Protoc. 2(5):1052-8. PDF
Chaulk SG, Kent, OA, MacMillan AM. (2005) Photo-control of RNA Processing" in Dynamic Studies in Biology: Phototriggers, Photoswitches, and Caged Biomolecules, (Goeldner, M. , Givens, R. Eds.) (Wiley-VCH Weinham, 2005) 495-513 PDF
Chaulk SG, MacMillan AM. (2001) Separation of Spliceosome Assembly from Catalysis with Caged pre-mRNA Substrates A.M.M. acknowledges support from the Natural Sciences and Engineering Research Council of Canada and the Alberta Heritage Foundation for Medical Research. Angew Chem Int Ed Engl. 40(11):2149-2152. PDF
Kent O, Chaulk SG, MacMillan AM. (2000) Kinetic analysis of the M1 RNA folding pathway. J Mol Biol. 304(5):699-705. PDF
Chaulk SG, Pezacki JP, MacMillan AM. (2000) Studies of RNA cleavage by photolysis of N-hydroxypyridine-2(1H)-thione. A new photochemical footprinting method. Biochemistry. 39(34):10448-53. PDF
Chaulk SG, MacMillan AM. (2000) Kinetic Footprinting of an RNA-Folding Pathway Using Peroxynitrous Acid. Angew Chem Int Ed Engl. 39(3):521-523. PDF
Chaulk SG, MacMillan AM. (2000) Characterization of the Tetrahymena ribozyme folding pathway using the kinetic footprinting reagent peroxynitrous acid. Biochemistry. 39(1):2-8. PDF
Chaulk SG, MacMillan AM. (1998) Caged RNA: photo-control of a ribozyme reaction. Nucleic Acids Res. 26(13):3173-8. PDF
Abstracts & Invited Seminars
Nicolas Coquelle
Nicolas Coquelle
Postdoctoral Fellow, 2009

Alberta Heritage Foundation for Medical Research Fellowship (2011-2014)

Aprataxin-like-factor (APLF) is a new member of the Non-Homologous End Joining (NHEJ) DNA repair pathway that interacts with XRCC4 and Ku80. My project focuses on a structural characterization of thoses protein complexes to probe further their role in the NHEJ pathway.

Jhuraney, A., Velkova, A., Johnson, R. C., Kessing, B., Carvalho, R. S., Whiley, P., Spurdle, A. B., Vreeswijk, M. P., Caputo, S. M., Millot, G. A., Vega, A., Coquelle, N., Galli, A., Eccles, D., Blok, M. J., Pal, T., van, d. e. r., Santamariña, P. e. n. a., Neuhausen, S. L., Donenberg, T., Machackova, E., Thomas, S., Vallée, M., Couch, F. J., Tavtigian, S. V., Glover, J. N., Carvalho, M. A., Brody, L. C., Sharan, S. K., Monteiro, A. N. (2015) BRCA1 Circos: a visualisation resource for functional analysis of missense variants. J Med Genet. 52(4):224-30. PDF
Awards

Alberta Heritage Foundation for Medical Research Fellowship (2011-2014)

Publications
Six during tenure in Glover's laboratory, eight total.
Jhuraney, A., Velkova, A., Johnson, R. C., Kessing, B., Carvalho, R. S., Whiley, P., Spurdle, A. B., Vreeswijk, M. P., Caputo, S. M., Millot, G. A., Vega, A., Coquelle, N., Galli, A., Eccles, D., Blok, M. J., Pal, T., van, d. e. r., Santamariña, P. e. n. a., Neuhausen, S. L., Donenberg, T., Machackova, E., Thomas, S., Vallée, M., Couch, F. J., Tavtigian, S. V., Glover, J. N., Carvalho, M. A., Brody, L. C., Sharan, S. K., Monteiro, A. N. (2015) BRCA1 Circos: a visualisation resource for functional analysis of missense variants. J Med Genet. 52(4):224-30. PDF
Coquelle, N., Glover, J. N. (2012) PARP pairs up to PARsylate. Nat Struct Mol Biol. 19(7):660-1. PDF
Coquelle, N., Havali-Shahriari, Z., Bernstein, N., Green, R., Glover, J. N. (2011) Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates. Proc Natl Acad Sci U S A. 108(52):21022-7. PDF
Coquelle, N., Green, R., Glover, J. N. M. (2011) Impact of BRCA1 BRCT Domain Missense Substitutions on Phosphopeptide Recognition. Biochemistry 50(21):4579-4589
Coquelle, N., Glover, J. N. M. (2010) FHA domain pThr binding specificity: it's all about me. Structure 18(12):1549-50.
Coquelle, N., Talon, R., Juers, D. H., Girard, E., Kahn, R., Madern, D. (2010) Gradual adaptive changes of a protein facing high salt concentrations. J Mol Biol. 404(3):493-505
Lee, M. S., Green, R., Marsillac, S. M., Coquelle, N., Williams, R. S., Yeung, T., Foo, D., Hau, D. D., Hui, B., Monteiro, A. N., Glover, J. N. M. (2010) Comprehensive analysis of missense variations in the BRCT domain of BRCA1 by structural and functional assays. Cancer Research 70(12):4880-4890. PDF
Coquelle N, Fioravanti E, Weik M, Vellieux F, Madern D. (2007) Activity, stability and structural studies of lactate dehydrogenases adapted to extreme thermal environments. J Mol Biol. 374(2):547-62. PDF
Abstracts & Invited Seminars
Ruth Green
Ruth Green
Technician, 1996

Coquelle, N., Havali-Shahriari, Z., Bernstein, N., Green, R., Glover, J. N. (2011) Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates. Proc Natl Acad Sci U S A. 108(52):21022-7. PDF
Awards
Publications
Six during tenure in Glover's laboratory, six total.
Coquelle, N., Havali-Shahriari, Z., Bernstein, N., Green, R., Glover, J. N. (2011) Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates. Proc Natl Acad Sci U S A. 108(52):21022-7. PDF
Coquelle, N., Green, R., Glover, J. N. M. (2011) Impact of BRCA1 BRCT Domain Missense Substitutions on Phosphopeptide Recognition. Biochemistry 50(21):4579-4589
Lee, M. S., Green, R., Marsillac, S. M., Coquelle, N., Williams, R. S., Yeung, T., Foo, D., Hau, D. D., Hui, B., Monteiro, A. N., Glover, J. N. M. (2010) Comprehensive analysis of missense variations in the BRCT domain of BRCA1 by structural and functional assays. Cancer Research 70(12):4880-4890. PDF
Williams, R. S., Bernstein, N. K., Lee, M. S., Rakovszky, M. L., Cui, D., Green, R., Weinfeld, M. and Glover, J. N. M. (2005) Structural basis for phosphorylation-dependent signaling in the DNA-damage response. Biochemistry and Cell Biology 83(6):721-727. PDF
Bernstein, N. K., Williams, R. S., Rakovszky, M. L., Cui, D., Green, R., Karimi-Busheri, F., Mani, R. S., Galicia, S. Koch, C. A., Cass, C. E., Durocher, D., Weinfeld, M. and Glover, J. N. M. (2005) The molecular architecture of the mammalian DNA repair enzyme, polynucleotide kinase. Molecular Cell 17(5):657-670. PDF
Williams, R. S., Green, R. and Glover, J. N. M. (2001) Crystal structure of the BRCT repeat region from the breast cancer-associated protein BRCA1. Nature Structural Biology 8(10):838-842. PDF
Abstracts & Invited Seminars
Jason Lamoureux
Jason Lamoureux
PhD, 1999-2006

Canada Graduate Scholarships Doctoral Award (2003-2005)

Structural investigations of Ndt80 in complex with DNA: Implications for DNA-protein interactions and transcriptional regulation.

Lamoureux, J. S. and Glover, J. N. M. (2006) Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes. Structure 14(3):555-565. PDF
Awards

Canada Graduate Scholarships Doctoral Award (2003-2005)

Faculty of Medicine and Dentistry 75th Anniversary Award 2000

AHFMR Studentship (2001-2006)

Publications
Three during tenure in Glover's laboratory, three total.
Lamoureux, J. S. and Glover, J. N. M. (2006) Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes. Structure 14(3):555-565. PDF
Lamoureux, J. S., Maynes, J. T. and Glover, J. N. M. (2004) Recognition of 5'-YpG-3' sequences by coupled stacking/hydrogen bonding interactions with amino acid residues. Journal Molecular Biology 335(2):399-408. PDF
Lamoureux, J. S., Stuart, D., Tsang, R., Wu, C. and Glover, J. N. M. (2002) Structure of the sporulation-specific transcription factor Ndt80 bound to DNA. The EMBO Journal 21:5721-5732. PDF
Abstracts & Invited Seminars
Megan Lee
Megan Lee
PhD, -2009

Investigation of the BRCT repeats in human hereditary breast cancer and DNA damage response.

Lee, M. S., Edwards, R. A., Thede, G. L., Glover, J. N. (2005) Structure of the BRCT repeat domain of MDC1 and its specificity for the free COOH-terminal end of the gamma-H2AX histone tail. J Biol Chem. 280(37):32053-6. PDF
Awards
Publications
Six during tenure in Glover's laboratory, six total.
Lee, M. S., Edwards, R. A., Thede, G. L., Glover, J. N. (2005) Structure of the BRCT repeat domain of MDC1 and its specificity for the free COOH-terminal end of the gamma-H2AX histone tail. J Biol Chem. 280(37):32053-6. PDF
Lee, M. S., Green, R., Marsillac, S. M., Coquelle, N., Williams, R. S., Yeung, T., Foo, D., Hau, D. D., Hui, B., Monteiro, A. N., Glover, J. N. (2010) Comprehensive analysis of missense variations in the BRCT domain of BRCA1 by structural and functional assays. Cancer Res. 70(12):4880-90. PDF
Edwards, R. A., Lee, M. S., Tsutakawa, S. E., Williams, R. S., Nazeer, I., Kleiman, F. E., Tainer, J. A., Glover, J. N. (2008) The BARD1 C-terminal domain structure and interactions with polyadenylation factor CstF-50. Biochemistry. 47(44):11446-56. PDF
Williams, R. S., Bernstein, N., Lee, M. S., Rakovszky, M. L., Cui, D., Green, R., Weinfeld, M., Glover, J. N. (2005) Structural basis for phosphorylation-dependent signaling in the DNA-damage response. Biochem Cell Biol. 83(6):721-7. PDF
Glover, J. N., Williams, R. S., Lee, M. S. (2004) Interactions between BRCT repeats and phosphoproteins: tangled up in two. Trends Biochem Sci. 29(11):579-85. PDF
Williams, R. S., Lee, M. S., Hau, D. D., Glover, J. N. (2004) Structural basis of phosphopeptide recognition by the BRCT domain of BRCA1. Nat Struct Mol Biol. 11(6):519-25. PDF
Abstracts & Invited Seminars
Charles Leung
Charles Leung
PhD, 2005-2011

Profiling Alberta Graduate Students Award (2010)

I work on the structural biology of TopBP1 in the DNA damage response. I am interested in characterizing protein-protein interactions that are critical for the role of TopBP1 in replication stress response.

Leung, C. C., Sun, L., Gong, Z., Burkat, M., Edwards, R., Assmus, M., Chen, J., Glover, J. N. (2013) Structural insights into recognition of MDC1 by TopBP1 in DNA replication checkpoint control. Structure. 21(8):1450-9. PDF
Awards

Profiling Alberta Graduate Students Award (2010)

Queen Elizabeth Doctoral Award (2008-2009)

CIHR Masters Award (2006-2007)

ACB Graduate Studentship (2006-2007)

Lionel B. Pett Graduate Scholarship in Biochemistry (2005)

Publications
Eight during tenure in Glover's laboratory, eight total.
Leung, C. C., Sun, L., Gong, Z., Burkat, M., Edwards, R., Assmus, M., Chen, J., Glover, J. N. (2013) Structural insights into recognition of MDC1 by TopBP1 in DNA replication checkpoint control. Structure. 21(8):1450-9. PDF
Campbell, S. J., Edwards, R. A., Leung, C. C., Neculai, D., Hodge, C. D., Dhe-Paganon, S., Glover, J. N. (2012) Molecular insights into the function of RING finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation. J Biol Chem. 287(28):23900-10. PDF
Leung, C. C., Glover, J. N. M. (2011) BRCT domains: easy as one, two, three. Cell Cycle 10(15):2461-2470
Liu, S., Shiotani, B., Lahiri, M., Maréchal, A., Tse, A., Leung, C. C., Glover, J. N. M., Yang, X. H., Zou, L. (2011) ATR autophosphorylation as a molecular switch for checkpoint activation. Molecular Cell 43(2):192-202
Leung, C. C. Y., Gong, Z., Chen, J., Glover, J. N. M. (2010) Molecular basis of BACH1/FANCJ recognition by TopBP1 in DNA replication checkpoint control. J Biol Chem. 2010 Dec 2. [Epub ahead of print]
Gong, Z., Kim, J.-E., Leung, C. C. Y., Glover, J. N. M. and Chen, J. (2010) BACH1/FANCJ acts with TopBP1 and participates early in DNA replication checkpoint control. Molecular Cell 37(3):438-446.
Leung, C. C. Y., Kellogg, E., Kuhnert, A., Hanel, F., Baker, D. and Glover, J. N. M. (2010) Insights from the crystal structure of the sixth BRCT domain of Topoisomerase II-β binding protein 1. Protein Science 19(1):162-167. PDF
Huang, J., Huen, M. S. Y., Kim, H., Leung, C. C. Y., Glover, J. N. M., Yu, X. and Chen, J. (2009) RAD18 transmits DNA damage signaling to elicit homologous recombination repair. Nature Cell Biology 11(5):592-603.
Abstracts & Invited Seminars
Inbal Mermershtain
Inbal Mermershtain (Greenberg)
Postdoctoral Fellow, 2010

I study the structure and function of the polynucleotide kinase/phosphatase (PNKP)/XRCC1/DNA Ligase III base excision repair (BER) complex.

Mermershtain, I., Glover, J. N. (2013) Structural mechanisms underlying signaling in the cellular response to DNA double strand breaks. Mutat Res. 750(1-2):15-22. PDF
Awards

Vatat Postdoc fellowship, The Sackler Faculty of Medicine, Tel Aviv University, Israel (2010)

Marian Gertner Institute for Medical Nanosystems Postdoc fellowship, The Sackler Faculty of Medicine, Tel Aviv University, Israel (2010)

Scholarship for Excellence, The Sackler Faculty of Medicine, Tel Aviv University, Israel (2003)

Publications
One during tenure in Glover's laboratory, six total.
Mermershtain, I., Glover, J. N. (2013) Structural mechanisms underlying signaling in the cellular response to DNA double strand breaks. Mutat Res. 750(1-2):15-22. PDF
Mermershtain I, Finarov I, Klipcan L, Kessler N, Rozenberg H, Safro MG. (2011) Idiosyncrasy and identity in the prokaryotic phe-system: crystal structure of E. coli phenylalanyl-tRNA synthetase complexed with phenylalanine and AMP. Protein Sci. 20(1):160-167
Auerbach T, Mermershtain I, Davidovich C, Bashan A, Belousoff M, Wekselman I, Zimmerman E, Xiong L, Klepacki D, Arakawa K, Kinashi H, Mankin AS, Yonath A (2010) The structure of the ribosome-lankacidin complex reveals ribosomal sites for synergistic antibiotics. Proc Natl Acad Sci U S A. 107:1983-1988
Auerbach T, Mermershtain I, Bashan A, Davidovich C, Rozenberg H, Sherman DH and Yonath A. (2009) Structural basis for the antibacterial activity of the 12-membered-ring mono-sugar macrolide methymycin. Biotechnologia 1(84):24-35
Greenberg I, Benayahu D. (2005) Osteo-Promoter Database (OPD) -- promoter analysis in skeletal cells. BMC Genomics 6:46
Agmon I, Amit M, Auerbach T, Bashan A, Baram D, Bartels H, Berisio R, Greenberg I, Harms J, Hansen HA, Kessler M, Pyetan E, Schluenzen F, Sittner A, Yonath A, Zarivach R. (2004) Ribosomal crystallography: a flexible nucleotide anchoring tRNA translocation, facilitates peptide-bond formation, chirality discrimination and antibiotics synergism. FEBS Lett. 567:20-26
Abstracts & Invited Seminars
Yun Peng
Yun Peng
Masters Student, 2010

My research is on the F-plasmid regulatory protein, TraJ.

Peng, Y., Lu, J., Wong, J. J., Edwards, R. A., Frost, L. S., Glover, J. N. (2014) Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM. J Mol Biol. 426(22):3783-95. PDF
Awards
Publications
Two during tenure in Glover's laboratory, two total.
Peng, Y., Lu, J., Wong, J. J., Edwards, R. A., Frost, L. S., Glover, J. N. (2014) Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM. J Mol Biol. 426(22):3783-95. PDF
Lu, J., Peng, Y., Arutyunov, D., Frost, L. S., Glover, J. N. (2012) Error-prone PCR mutagenesis reveals functional domains of a bacterial transcriptional activator, TraJ. J Bacteriol. () PDF
Abstracts & Invited Seminars
Barbara Pedrycz
Barbara Pedrycz
Masters Student, 2012

Awards
Publications
0 during tenure in Glover's laboratory, 0 total.
Abstracts & Invited Seminars
Gina Thede
Gina Thede
PhD, 2004-2012

Frederick Banting and Charles Best Canada Graduate Scholarship

My project focuses on the biochemical and structural characterization of CpxP, a periplasmic protein that regulates the Cpx envelope stress response system in Gram-negative bacteria such as E. coli.

Thede, G. L., Arthur, D. C., Edwards, R. A., Buelow, D. R., Wong, J. L., Raivio, T. L., Glover, J. N. M. (2011) Structure of the periplasmic stress response protein CpxP. J Bacteriol. 2011 Feb 11. [Epub ahead of print]
Awards

Frederick Banting and Charles Best Canada Graduate Scholarship

President's Doctoral Prize of Distinction (2010)

AHFMR Studentship (2006-2011)

CIHR Canada Graduate Scholarship Masters Award 2005

Publications
Three during tenure in Glover's laboratory, three total.
Thede, G. L., Arthur, D. C., Edwards, R. A., Buelow, D. R., Wong, J. L., Raivio, T. L., Glover, J. N. M. (2011) Structure of the periplasmic stress response protein CpxP. J Bacteriol. 2011 Feb 11. [Epub ahead of print]
Chaulk, S. G., Thede, G. L., Kent, O. A., Xu, Z., Gesner, E., Veldhoen, R. A., Khanna, S. K., Goping, I. S., Macmillan, A. M., Mendell, J. T., Young, H., Fahlman, R. P., Glover, J. N. M. (2011) Role of pri-miRNA tertiary structure in miR-17~92 miRNA biogenesis. RNA Biology 8(6). [Epub ahead of print]
Lee, M. S., Edwards, R. A., Thede, G. L. and Glover, J. N. M. (2005) Structure of the BRCT repeat domain of MDC1 and its specificity for the free COOH-terminal end of the gamma-H2AX histone tail. JBC 280(37):32053-32056. PDF
Abstracts & Invited Seminars
Scott Williams
Scott Williams
PhD, -2003

Structural Studies of the BRCA1 BRCT Domain.

Lee, M. S., Green, R., Marsillac, S. M., Coquelle, N., Williams, R. S., Yeung, T., Foo, D., Hau, D. D., Hui, B., Monteiro, A. N., Glover, J. N. (2010) Comprehensive analysis of missense variations in the BRCT domain of BRCA1 by structural and functional assays. Cancer Res. 70(12):4880-90. PDF
Awards
Publications
12 during tenure in Glover's laboratory, 12 total.
Lee, M. S., Green, R., Marsillac, S. M., Coquelle, N., Williams, R. S., Yeung, T., Foo, D., Hau, D. D., Hui, B., Monteiro, A. N., Glover, J. N. (2010) Comprehensive analysis of missense variations in the BRCT domain of BRCA1 by structural and functional assays. Cancer Res. 70(12):4880-90. PDF
Edwards, R. A., Lee, M. S., Tsutakawa, S. E., Williams, R. S., Nazeer, I., Kleiman, F. E., Tainer, J. A., Glover, J. N. (2008) The BARD1 C-terminal domain structure and interactions with polyadenylation factor CstF-50. Biochemistry. 47(44):11446-56. PDF
Simons, A. M., Horwitz, A. A., Starita, L. M., Griffin, K., Williams, R. S., Glover, J. N., Parvin, J. D. (2006) BRCA1 DNA-binding activity is stimulated by BARD1. Cancer Res. 66(4):2012-8. PDF
Williams, R. S., Bernstein, N., Lee, M. S., Rakovszky, M. L., Cui, D., Green, R., Weinfeld, M., Glover, J. N. (2005) Structural basis for phosphorylation-dependent signaling in the DNA-damage response. Biochem Cell Biol. 83(6):721-7. PDF
Bernstein, N. K., Williams, R. S., Rakovszky, M. L., Cui, D., Green, R., Karimi-Busheri, F., Mani, R. S., Galicia, S., Koch, C. A., Cass, C. E., Durocher, D., Weinfeld, M., Glover, J. N. (2005) The molecular architecture of the mammalian DNA repair enzyme, polynucleotide kinase. Mol Cell. 17(5):657-70. PDF
Glover, J. N., Williams, R. S., Lee, M. S. (2004) Interactions between BRCT repeats and phosphoproteins: tangled up in two. Trends Biochem Sci. 29(11):579-85. PDF
Dubin, M. J., Stokes, P. H., Sum, E. Y., Williams, R. S., Valova, V. A., Robinson, P. J., Lindeman, G. J., Glover, J. N., Visvader, J. E., Matthews, J. M. (2004) Dimerization of CtIP, a BRCA1- and CtBP-interacting protein, is mediated by an N-terminal coiled-coil motif. J Biol Chem. 279(26):26932-8. PDF
Williams, R. S., Lee, M. S., Hau, D. D., Glover, J. N. (2004) Structural basis of phosphopeptide recognition by the BRCT domain of BRCA1. Nat Struct Mol Biol. 11(6):519-25. PDF
Williams, R. S., Chasman, D. I., Hau, D. D., Hui, B., Lau, A. Y., Glover, J. N. (2003) Detection of protein folding defects caused by BRCA1-BRCT truncation and missense mutations. J Biol Chem. 278(52):53007-16. PDF
Williams, R. S., Glover, J. N. (2003) Structural consequences of a cancer-causing BRCA1-BRCT missense mutation. J Biol Chem. 278(4):2630-5 PDF
Hamilton, K. S., Ellison, M. J., Barber, K. R., Williams, R. S., Huzil, J. T., McKenna, S., Ptak, C., Glover, M., Shaw, G. S. (2001) Structure of a conjugating enzyme-ubiquitin thiolester intermediate reveals a novel role for the ubiquitin tail. Structure. 9(10):897-904 PDF
Williams, R. S., Green, R., Glover, J. N. (2001) Crystal structure of the BRCT repeat region from the breast cancer-associated protein BRCA1. Nat Struct Biol. 8(10):838-842 PDF
Abstracts & Invited Seminars
Joyce Wong
Joyce Wong
PhD, 2005-2012

Frederick Banting and Charles Best Canada Graduate Scholar (2009-2011)

I work on TraM of F-like plasmids, a protein essential for conjugative DNA transfer. My projects are the characterization of TraM interaction with DNA at the plasmid origin of transfer region, and the interaction of TraM with TraD, a protein which is part of the transmembrane complex that mediates DNA transfer.

Peng, Y., Lu, J., Wong, J. J., Edwards, R. A., Frost, L. S., Glover, J. N. (2014) Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM. J Mol Biol. 426(22):3783-95. PDF
Awards

Frederick Banting and Charles Best Canada Graduate Scholar (2009-2011)

President's Doctoral Prize of Distinction (2010)

Faculty of Medicine and Dentistry - 75th Anniversary Award (2006)

Publications
Five during tenure in Glover's laboratory, five total.
Peng, Y., Lu, J., Wong, J. J., Edwards, R. A., Frost, L. S., Glover, J. N. (2014) Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM. J Mol Biol. 426(22):3783-95. PDF
Wong, J. J., Lu, J., Glover, J. N. (2012) Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. PDF
Wong, J. J., Lu, J., Edwards, R. A., Frost, L. S., Glover, J. N. M. (2011) Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM. Nucleic Acids Research Epub ahead of print
Lu, J., Wong, J. J. W., Edwards, R. A., Manchak, J., Frost, L. S. and Glover, J. N. M. (2008) Structural basis of specific TraD-TraM recognition during F plasmid-mediated bacterial conjugation. Molecular Microbiology 70(1):89-99. PDF
Lu, J., Edwards, R. A., Wong, J. J. W., Manchak, J. Scott, P. G., Frost, L. S. and Glover, J. N. M. (2006) Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM. EMBO Journal 25(12):2930-2939. PDF
Abstracts & Invited Seminars
Yeyun Zhou
Yeyun Zhou
Postdoctoral Fellow, 2013

AIHS Postdoctoral Fellowship (2013-2016)

Activity of p53, the major human tumor suppressor, is regulated by the serine/threonine phosphatase, PP1. PP1 dephosphorylates p53 via interactions with members of the ASPP (Apoptotic Stimulating Proteins of p53) family. My project focuses on the structural and biochemical characterization of the interactions of PP1/ASPP/p53 complexes, with an ultimate goal to develop novel anti-cancer drug leads.

Du, J., Zhou, Y., Su, X., Yu, J. J., Khan, S., Jiang, H., Kim, J., Woo, J., Kim, J. H., Choi, B. H., He, B., Chen, W., Zhang, S., Cerione, R. A., Auwerx, J., Hao, Q., Lin, H. (2011) Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase. Science. 334(6057):806-9.
Awards

AIHS Postdoctoral Fellowship (2013-2016)

Publications
0 during tenure in Glover's laboratory, seven total.
Du, J., Zhou, Y., Su, X., Yu, J. J., Khan, S., Jiang, H., Kim, J., Woo, J., Kim, J. H., Choi, B. H., He, B., Chen, W., Zhang, S., Cerione, R. A., Auwerx, J., Hao, Q., Lin, H. (2011) Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase. Science. 334(6057):806-9.
Zhu, A. Y., Zhou, Y., Khan, S., Deitsch, K. W., Hao, Q., Lin, H. (2012) Plasmodium falciparum Sir2A preferentially hydrolyzes medium and long chain fatty acyl lysine. ACS Chem Biol. 7(1):155-9.
Zhou, Y., Zhang, H., He, B., Du, J., Lin, H., Cerione, R. A., Hao, Q. (2012) The bicyclic intermediate structure provides insights into the desuccinylation mechanism of human sirtuin 5 (SIRT5). J Biol Chem. 287(34):28307-14.
Dong, M., Si, Y. Q., Sun, S. Y., Pu, X. P., Yang, Z. J., Zhang, L. R., Zhang, L. H., Leung, F. P., Lam, C. M., Kwong, A. K., Yue, J., Zhou, Y., Kriksunov, I. A., Hao, Q., Lee, H. C. (2011) Design, synthesis and biological characterization of novel inhibitors of CD38. Org Biomol Chem. 9(9):3246-57.
Guo, P. C., Zhou, Y. Y., Ma, X. X., Li, W. F. (2010) Structure of Hsp33/YOR391Cp from the yeast Saccharomyces cerevisiae. Acta Crystallogr Sect F Struct Biol Cryst Commun. 66(Pt):12.
He, Y. X., Gui, L., Liu, Y. Z., Du, Y., Zhou, Y., Li, P., Zhou, C. Z. (2009) Crystal structure of Saccharomyces cerevisiae glutamine synthetase Gln1 suggests a nanotube-like supramolecular assembly. Proteins. 76(1):249-54.
Liu, W., Zhou, Y. Y., Teng, M. K., Zhou, C. Z. (2007) Purification, crystallization and preliminary X-ray analysis of Hsp33 from Saccharomyces cerevisiae. Acta Crystallogr Sect F Struct Biol Cryst Commun. 63(Pt):2.
Abstracts & Invited Seminars